Working with Genetically Modified Mice in Research Settings

Discusses the basic concepts and history of working with genetically modified mice.

Recommended Use: Required
ID (Language): 19272 (English)
Author(s): Jennifer Kalishman, DVM, MS - Washington University School of Medicine

Describes how the use of standard nomenclature helps the scientific community share relevant information associated with each mutation and benefit from the reported animal model. The module provides information on standard nomenclature for gene symbols and gene names, how to use the Mouse Genome Informatics (MGI) resource, the usefulness and standardization of laboratory codes, differences in strains, and common inbred parental strains and substrains.

Recommended Use: Required
ID (Language): 19273 (English)
Author(s): Jennifer Kalishman, DVM, MS - Washington University School of Medicine

Provides an overview of genetic engineering for generating genetically modified mouse models. The module discusses the significance of genetically modified mouse models, the types of cloning vectors, what is meant by a “transgene,” basic steps in engineering cells, differences between blastocyst and pronuclear embryo injections, and various gene editing techniques such as CRISPR-Cas9.

Recommended Use: Required
ID (Language): 19274 (English)
Author(s): Jennifer Kalishman, DVM, MS - Washington University School of Medicine

Explains the process for breeding and maintenance of genetically modified mice. The module provides information on mouse repositories, the timeline for transgenic projects, the importance of choosing a background strain, how founder animals are established, backcrossing, techniques for identifying animals, steps involved in genotyping, the importance of designing good primers, breeding schemes and maintaining transgenic strains, Cre/lox technology, and how to optimize breeding performance.

Recommended Use: Required
ID (Language): 19275 (English)
Author(s): Jennifer Kalishman, DVM, MS - Washington University School of Medicine

Describes the animal welfare and regulatory issues and guidelines associated with the production of genetically modified mice. The module discusses monitoring genetically modified mice for clinical issues and reporting animal welfare issues to the veterinary staff and IACUC.

Recommended Use: Required
ID (Language): 19276 (English)
Author(s): Jennifer Kalishman, DVM, MS - Washington University School of Medicine