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Working with Genetically Modified Mice in Research Settings

This course provides training on working with genetically modified mice in research settings.

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About this Course


This course offers an introduction to working with genetically modified mice in research settings, including basic concepts and history, nomenclature, genetic engineering, breeding and maintenance, and animal welfare and regulations.

Language Availability: English

Suggested Audiences: IACUC Members, Animal Workers, Investigators, Research Assistants, Staff

Organizational Subscription Price: Included in organizational subscription
Independent Learner Price: $99 per person


Course Content


Basic Concepts and History New Content

Discusses the basic concepts and history of working with genetically modified mice.

Recommended Use: Required
ID (Language): 19272 (English)
Author(s): Jennifer Kalishman, DVM, MS - Washington University School of Medicine

Nomenclature New Content

Describes how the use of standard nomenclature helps the scientific community share relevant information associated with each mutation and benefit from the reported animal model. The module provides information on standard nomenclature for gene symbols and gene names, how to use the Mouse Genome Informatics (MGI) resource, the usefulness and standardization of laboratory codes, differences in strains, and common inbred parental strains and substrains.

Recommended Use: Required
ID (Language): 19273 (English)
Author(s): Jennifer Kalishman, DVM, MS - Washington University School of Medicine

Genetic Engineering New Content

Provides an overview of genetic engineering for generating genetically modified mouse models. The module discusses the significance of genetically modified mouse models, the types of cloning vectors, what is meant by a “transgene,” basic steps in engineering cells, differences between blastocyst and pronuclear embryo injections, and various gene editing techniques such as CRISPR-Cas9.

Recommended Use: Required
ID (Language): 19274 (English)
Author(s): Jennifer Kalishman, DVM, MS - Washington University School of Medicine

Breeding and Maintenance New Content

Explains the process for breeding and maintenance of genetically modified mice. The module provides information on mouse repositories, the timeline for transgenic projects, the importance of choosing a background strain, how founder animals are established, backcrossing, techniques for identifying animals, steps involved in genotyping, the importance of designing good primers, breeding schemes and maintaining transgenic strains, Cre/lox technology, and how to optimize breeding performance.

Recommended Use: Required
ID (Language): 19275 (English)
Author(s): Jennifer Kalishman, DVM, MS - Washington University School of Medicine

Animal Welfare and Regulations New Content

Describes the animal welfare and regulatory issues and guidelines associated with the production of genetically modified mice. The module discusses monitoring genetically modified mice for clinical issues and reporting animal welfare issues to the veterinary staff and IACUC.

Recommended Use: Required
ID (Language): 19276 (English)
Author(s): Jennifer Kalishman, DVM, MS - Washington University School of Medicine