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Working with Zebrafish (Danio rerio) in Research Settings

This course provides training on working with zebrafish (danio rerio) in research settings.

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About this Course


This course offers an introduction to working with zebrafish (danio rerio) in research settings including the regulatory mandates and biological features of zebrafish (danio rerio).

Language Availability: English

Suggested Audiences: IACUC Members, Animal Workers, Investigators, Research Assistants, Staff


Course Content


Toggle Content Introduction, Background, Research Mandates, Zoonotic Diseases and Occupational Health Considerations

Provides an overview of zebrafish and the advantages of using zebrafish as a research model. It also covers the federal mandates that regulate the use of zebrafish in research, the need for an occupational health and safety program, and the potential hazards that come with handling zebrafish.

Recommended Use: Required
ID (Language): 16243 (English)

Toggle Content Humane Standards, Procurement and Quarantine, and Identification

Explains the need for humane standards in research. It also details the process for obtaining, transporting, and quarantining zebrafish.

Recommended Use: Required
ID (Language): 16244 (English)

Toggle Content Husbandry, Microenvironment, Nutrition, and Animal Records

Discusses the water treatment, conditioning, and flow requirements for a zebrafish facility.  It also discusses primary enclosures, biosecurity, environmental conditions, nutritional needs, and proper record keeping for animals and facilities.

Recommended Use: Required
ID (Language): 16245 (English)

Toggle Content Biological and Behavioral Features, the Zebrafish Genome, Reproduction and Development

Details zebrafish anatomy, physiology, and behavior. It also describes the zebrafish genome and stages of development, as well as reproduction including breeding schemes and development.

Recommended Use: Required
ID (Language): 16246 (English)

Toggle Content Recognizing Pain and Distress, Restraint, Anesthesia and Sedation, Surgery and Euthanasia

Describes how to detect pain and distress in zebrafish, as well as the types of anesthetic and analgesic agents used in zebrafish and the indications for their use. It also discusses the factors that should be taken into consideration when performing surgery on zebrafish and presents the acceptable methods of euthanasia in zebrafish and for their disposal.

Recommended Use: Required
ID (Language): 16247 (English)