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Initial Biosafety Training

This course offers initial training for researchers handling biohazards in a research or clinical laboratory.


About this Course


Initial Biosafety Training provides initial training for researchers handling biohazards in a research or clinical laboratory. The course addresses awareness of biohazards, risk assessment, and key risk management principles including work practices, personal protective equipment (PPE), engineering controls, and emergency response.

Language Availability: English

Suggested Audiences: Researchers Working in a Laboratory

Organizational Subscription Price: Included in additional $500 annual subscription fee for Biosafety and Biosecurity
Independent Learner Price: $110 per person


Course Content


Toggle Content Biosafety Course Overview

Provides an introduction to biosafety and training for researchers handling Risk Group 1 agents and working only at Biosafety Level 1 (BSL-1) containment.

Recommended Use: Required
ID (Language): 13314 (English)
Author(s): Benjamin Fontes, MPH, CBSP - Yale University

Toggle Content Laboratory-Acquired Infections

Sets the foundation for the importance of biosafety by briefly reviewing seminal laboratory-acquired infections that have occurred in the research and clinical laboratory settings. A learner may explore supplemental cases for further study and increase his/her awareness of how the breakdown of good microbiological practices can lead to infections and death (in rare cases).

Recommended Use: Required
ID (Language): 13454 (English)
Author(s): Benjamin Fontes, MPH, CBSP - Yale University

Toggle Content Biohazard Risk Assessment

Outlines the steps involved in identifying risk factors for experiments or procedures involving biohazards. The three Ps of risk assessment (Pathogen, Procedures, and Personnel) are reviewed to provide a basic foundation in this important building block to biosafety.

Recommended Use: Required
ID (Language): 13455 (English)
Author(s): Benjamin Fontes, MPH, CBSP - Yale University

Toggle Content Medical Surveillance

Reviews basic medical surveillance and occupational health program recommendations for laboratory or clinical researchers handling biohazards.

Recommended Use: Required
ID (Language): 13456 (English)
Author(s): Benjamin Fontes, MPH, CBSP - Yale University

Toggle Content Risk Management: Work Practices

Serves as initial training targeted at researchers handling or who will handle biohazards in a research or clinical laboratory. It addresses an awareness of biohazards, risk assessment, and key risk management work practice principles.

Recommended Use: Required
ID (Language): 13898 (English)
Author(s): Benjamin Fontes, MPH, CBSP - Yale University

Toggle Content Risk Management: Personal Protective Equipment

Discusses U.S. Occupational Safety and Health Administration (OSHA) personal protective equipment (PPE) training requirements for researchers handling biohazards. A supplemental PPE Training Record Form and PPE Training and Use Table are available at the end to facilitate the documentation of training.

Recommended Use: Required
ID (Language): 13458 (English)
Author(s): Benjamin Fontes, MPH, CBSP - Yale University

Toggle Content Risk Management: Emergency and Spill Response

Provides biohazard emergency response training for employees who are designated as hazardous materials responders and address the biohazard component of their annual training.

Recommended Use: Required
ID (Language): 13459 (English)
Author(s): Benjamin Fontes, MPH, CBSP - Yale University

Toggle Content Risk Management: Engineering Controls

Examines the engineering controls, devices, and barriers that can be utilized to protect researchers when handling biohazards in the laboratory. It also describes the fundamental engineering controls utilized to safely handle biohazards and the concomitant work practices for their safe use.

Recommended Use: Required
ID (Language): 13929 (English)
Author(s): Benjamin Fontes, MPH, CBSP - Yale University

Toggle Content Risk Management: Laboratory Design

Describes the safety features of laboratories designed to protect researchers at each of the first three Biosafety Levels (1, 2, and 3). It provides a comprehensive description of the safety factors associated with the design of a Biosafety Level 3 (BSL-3) laboratory.

Recommended Use: Required
ID (Language): 13484 (English)
Author(s): Benjamin Fontes, MPH, CBSP - Yale University

Toggle Content Work Safely with Sharp Instruments

Reviews how to safely handle sharp instruments when working with biohazards. It outlines safe work practices with disposable and reusable sharps.

Recommended Use: Required
ID (Language): 13899 (English)
Author(s): Benjamin Fontes, MPH, CBSP - Yale University

Toggle Content Disinfection and Sterilization

Discusses the decontamination of items contaminated by biohazards after an experiment, prior to reuse, or before disposal. It also provides information on methods of sterilization and disinfection, along with good decontamination work practices.

Recommended Use: Required
ID (Language): 13900 (English)
Author(s): Benjamin Fontes, MPH, CBSP - Yale University

Toggle Content Safe Sharps Devices

Provides an overview of safe sharps devices and other engineering controls designed to minimize exposure to biohazards when handling sharp instruments.  It also supports training on a sharps safety program.

Recommended Use: Required
ID (Language): 13946 (English)
Author(s): Benjamin Fontes, MPH, CBSP - Yale University

Toggle Content Centrifuge Precautions

Discusses risks involved in centrifugation along with the control devices and work practices that will help ensure the safe use of centrifuges.

Recommended Use: Required
ID (Language): 13945 (English)
Author(s): Benjamin Fontes, MPH, CBSP - Yale University

Toggle Content Engineering Controls and Containment Devices

Describes a variety of engineering controls or the protective tools and devices used in the laboratory setting that help protect workers from exposure to biohazards.

Recommended Use: Required
ID (Language): 13497 (English)
Author(s): Benjamin Fontes, MPH, CBSP - Yale University

Toggle Content Dual Use Research of Concern (DURC)

Detailed training on the United States Government Policy for Institutional Oversight of Life Sciences Dual Use Research of Concern. It is based on the 2014 United States Government Policy for Oversight of Life Sciences Dual Use Research of Concern, which is required for all National Institutes of Health (NIH) funded entities. It describes the agents and types of experiments that require oversight, individual and organizational responsibilities, and risk mitigation strategies.

Recommended Use: Supplemental
ID (Language): 16263 (English)
Author(s): Angela Birnbaum, MPH - Tulane University

Toggle Content Biosafety and Biosecurity (BSS) Introduction

Introduces CITI Program’s Biosafety and Biosecurity (BSS) series.

Recommended Use: Supplemental
ID (Language): 13987 (English)